High cell density perfusion cultures of anchorage-dependent Vero cells in a depth filter perfusion system

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Title
High cell density perfusion cultures of anchorage-dependent Vero cells in a depth filter perfusion system
Author(s)
Sang Kyo Choi; Ho Nam Chang; Gyun Min Lee; Ik Hwan Kim; Duk Jae Oh
Bibliographic Citation
Cytotechnology, vol. 17, pp. 173-183
Publication Year
1995
Abstract
A depth filter perfusion system (DFPS) with polypropylene fibers had been demonstrated to support high density cultures of anchorage-independent hybridoma cells. The DFPS provides advantages of high surface-to-volume ratio of 450-600 cm(2)/cm(3), low cost set-up, easy operation and scale-up. To test the feasibility of using DFPS for high density cultures of anchorage-dependent cells, Vero cells were cultivated in the DFPS. Gelatin coating on polypropylene fibers in the DFPS was necessary to promote cell attachment and growth. Dissolved oxygen (DO) concentrations could be controlled by sparging air into the reservoir vessel through a filter sparger. When DO concentration was controlled above 40% of air saturation in the DFPS with 40 μm pore size, the maximum cell concentration as estimated on specific lactate production rate, was 3.81×10(7) cells/ml of the total reactor volume. This viable cell concentration is approximately 18 times higher than that obtained in a T-flask batch culture. Taken together, the results obtained here showed the potential of DFPS for high-density cultures of anchorage-dependent cells
Keyword
polypropylene fiberair sparginghigh cell density culturedepth filter perfusion systemVero cell culturegelatin coating
ISSN
0920-9069
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00749655
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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