Description of Olsenella absiana sp. nov., an anaerobic bacterium isolated from pig faeces

Abstract

A short-rod-shaped, obligate anaerobic, Gram-positive bacterium isolated from the faeces of a pig was designated as the strain YH-ols2223T. Analysis of the 16S rRNA gene sequence revealed that the isolate was most closely related to Olsenella urininfantis KCTC 25755T with 97.7% similarity. The multi-locus sequence tree revealed that the isolate formed a sub-cluster within the genus Olsenella. The average nucleotide identity and the digital DNA-DNA hybridization values for the isolate and the most closely related strains were 74.9-76.6% and 20.8-22.5%, respectively. The end products of the fermentation were lactic acid and acetic acid. The main fatty acids identified were C18 : 0 DMA, C18 : 1 ω9c DMA and C18 : 1 ω9c. The cell wall contained the meso-diaminopimelic acid-based peptidoglycan. Given the chemotaxonomic, phenotypic and phylogenetic properties, YH-ols2223T (= KCTC 25800T = NBRC 116680T) represents a novel taxon. The name Olsenella absiana sp. nov. was proposed.