Characterization of cell lines established from human hepatocellular carcinoma

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Title
Characterization of cell lines established from human hepatocellular carcinoma
Author(s)
Jae-Gahb Park; Jae-Ho Lee; Young Ik Lee; Yong-Il Kim
Bibliographic Citation
International Journal of Cancer, vol. 62, no. 3, pp. 276-282
Publication Year
1995
Abstract
We characterized 8 human hepatocellular-carcinoma cell lines established from the primary tumors of Korean patients. All lines showed substrate adherence and one line from anaplastic tumor also grew as floating aggregates. Most cultured cells maintained many morphological characteristics of the original tumors from which they were derived. Doubling times varied from 34 to 72 hr. All lines showed relatively high viability and were not contaminated with Mycoplasma or bacteria. All lines showed aneuploidy and were proven to be unique by DNA fingerprinting analysis. Hepatitis-B-virus (HBV) DNA was integrated in the genomes of all lines. Two of the cell lines (SNU-354, SNU-368) showed expression of HBV and HBVx (HBx) transcripts. SNU-354 strongly expressed albumin, and SNU-368 expressed transferrin and insulin-like growth factor II. No lines produced alpha-fetoprotein at the RNA and protein level. These cell lines represent useful tools for in vitro studies related to hepatocellular carcinoma.
Keyword
cancer cell culturehumanhuman cellliver cell carcinomacarcinoma, hepatocellularcell divisiontumor cells, cultured
ISSN
0020-7136
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/ijc.2910620308
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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