Roseateles microcysteis sp. nov., Isolated from Co-cultivation of Microcystis aeruginosa and Myriophyllum spicatum

Abstract

A novel rod-shaped, Gram-stain-negative bacterial strain MS17T was obtained from a co-culture of Microcystis aeruginosa and Myriophyllum spicatum. The examination of the 16S ribosomal RNA gene sequence showed a significant degree of similarity between strain MS17T and Paucibacter sediminis S2-9T (98.4%), Roseateles violae PFR6T (98.1%), ‘Roseateles cellulosilyticus’ P8T (98.0%), Roseateles aquae APW11T (97.9%), Roseateles oligotrophus CHU3T (97.7%), Roseateles saccharophilus DSM 654T (97.6%), Kinneretia aquatilis CR182T (97.7%), Pelomonas aquatica CCUG 52575T (97.6%), and Roseateles toxinivorans 2C20T (97.0%). Between strain MS17T and the type strains of closely related species, digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were below 21.9% and 78.48%. The respiratory quinone was ubiquinone Q-8. The main fatty acids (>？10.0%) were C15:1 ω6c, C16:0, C17:0, and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The polar lipids comprised phosphatidylethanolamine, unidentified phospholipid, phosphatidylglycerol, unidentified aminophospholipid, unidentified aminolipid, and three unidentified lipids. The genomic G？+？C content was 65.9%. According to phenotypic characteristics, phylogenetic relationships, and chemotaxonomic data, strain MS17T has been categorized as a newly discovered species belonging to the Roseateles genus, and the name Roseateles microcysteis sp. nov. is suggested. It exhibits distinct biochemical properties that differentiate it from closely related species. The type strain is MS17T (=KCTC 8001T？=？LMG 33142T).