A Novel Aerobic Respiratory Chain-Linked NADH Oxidase System in Zymomonas mobilis

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Title
A Novel Aerobic Respiratory Chain-Linked NADH Oxidase System in Zymomonas mobilis
Author(s)
YOUNG JAE KIM; Ki Bang Song; Sang Ki Rhee
Bibliographic Citation
Journal of Bacteriology, vol. 177, no. 17, pp. 5176-5178
Publication Year
1995
Abstract
Membrane vesicles prepared from Zymomonas mobilis oxidized NADH exclusively, whereas deamino-NADH was little oxidized. In addition, the respiratory chain-linked NADH oxidase system exhibited only a single apparent K(m) value of approximately 66 μM for NADH. The NADH oxidase was highly sensitive to the respiratory chain inhibitor 2-heptyl-4-hydroxyquinoline-N- oxide. However, the NADH:quinone oxidoreductase was not sensitive to 2- heptyl-4-hydroxyquinoline-N-oxide and was highly resistant to another respiratory chain inhibitor, rotenone. Electron transfer from NADH to oxygen generated a proton electrochemical gradient (inside positive) in inside-out membrane vesicles. In contrast, electron transfer from NADH to ubiquinone-1 generated no electrochemical gradient. These findings indicate that Z. mobilis possesses only NADH:quinone oxidoreductase lacking the energy coupling site.
ISSN
0021-9193
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/jb.177.17.5176-5178.1995
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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