Maximum yield of foreign lipase in Escherichia coli HB101 limited by duration of protein expression

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Title
Maximum yield of foreign lipase in Escherichia coli HB101 limited by duration of protein expression
Author(s)
함대현; SH Kim; Jae Gu Pan; JS Rhee
Bibliographic Citation
Journal of Fermentation Bioengineering, vol. 79, no. 3, pp. 236-241
Publication Year
1995
Abstract
When a microbial lipase was overexpressed in Escherichia coli HB101, the expression kinetics as represented by the expression rate, duration, and maximum yield of lipase were studied. Lipase synthesis, controlled by the tac promoter, continued for about 4 h after IPTG induction. The duration of the expression phase was similar, irrespective of expression rate and yield, which were manipulated by using a-methyl glucose (α-MG), a competitive inhibitor of glucose. By measuring the specific oxygen uptake rate, specific CO2 evolution rate, specific glucose uptake rate, intracellular protease level and the acetate concentration in the culture, the limited duration of the expression phase was found to be caused by metabolic stress arising from the rapid and massive production of the foreign protein under the strong promoter. Neither the total cell number nor the number of living cells increased substantially after induction, whereas the optical density of the culture gradually increased. The duration of the expression phase was reduced to less than 2 h by the addition of menadione, a redox cycling agent, seemingly due to an acceleration of the energetic flow of the host cells after induction. In contrast, the duration of the expression phase was extended to 8 h in the glucose-starved condition, although the maximum expression yield was much lower than that in the glucose-surplus condition. Therefore, it was suggested that the expression rate after induction determined the maximum expression yield of the foreign lipase gene in E. coli HB101 because of the restrained capacity of foreign protein production.
Keyword
escherichia coilforeign lipase yieldlimitationprotein expressiontriacylglycerol lipaseescherichia coligene expression
ISSN
0385-6380
Publisher
Soc Bioscience Bioengineering Japan
DOI
http://dx.doi.org/10.1016/0922-338X(95)90609-4
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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