An interchangeable system of hairy root and cell suspension cultures of Catharanthus roseus for indole alkaloid production

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Title
An interchangeable system of hairy root and cell suspension cultures of Catharanthus roseus for indole alkaloid production
Author(s)
Jung KH; Sang Soo Kwak; Cha Yong Choi; Jang Ryol Liu
Bibliographic Citation
Plant Cell Reports, vol. 15, pp. 51-54
Publication Year
1995
Abstract
Hairy roots ofCatharanthus roseus obtained by co-cultivation of hypocotyl segments withAgrobacterium rhizogenes, and cultured in SH (Schenk and Hildebrandt) basal medium, formed two types of calli when subcultured in SH medium with 1 mg/1 -naphthaleneacetic acid and 0.1 mg/l kinetin. One of them, a compact callus, when re-subcultured in SH basal medium gave rise to hairy roots again. A rhizogenic cell suspension culture was established from this type of callus. When cultured in SH medium with growth regulators, the rhizogenic callus produced catharanthine at a level of 41% of the level in the initial hairy roots. Upon transfer to SH basal medium, regenerated hairy roots produced this alkaloid at the original level of 1.5 mg/g dry wt. Using this cell/hairy root interchange system a new management system for hairy root culture in bioreactors has been devised and examined involving production of biomass in the form of a cell suspension in medium supplemented with growth regulators, and catharanthine production by hairy roots regenerated from these cells in medium without growth regulators.
Keyword
CatharanthineCatharanthus roseusHairy rootsRhizogenic callus
ISSN
0721-7714
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF01690252
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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