Inhibition of PDGF-induced phospholipase D but not phospholipase C activation by herbimycin A

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Title
Inhibition of PDGF-induced phospholipase D but not phospholipase C activation by herbimycin A
Author(s)
Bo Yeon Kim; Soon Cheol Ahn; 오혜경; Hyun Sun Lee; 민태익; Hyune Mo Rho; Jong Seog Ahn
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 212, no. 3, pp. 1061-1067
Publication Year
1995
Abstract
It is known that PDGF-induced phospholipase D (PLD) activation occurs downstream of phospholipase Cγ1 (PLC-γ1) activation. Herbimycin A, a specific inhibitor of Src family tyrosine kinase, dose-dependently inhibited PDGF-induced PLD activation (IC50 of 8 μg/ml) without affecting PLC-γ1 activation and significantly reduced PDGF-induced tyrosine phosphorylations of 40-46 kD and 60 kD proteins in PLC-γ1-overexpressing NIH 3T3γ1 cells. These results suggest that PLC activation is necessary but not sufficient for PDGF-induced PLD activation. Tyrosine phosphorylation of p40-p46 and p60 is thought to be involved in PDGF-induced activation of PLD but not of PLC.
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1006/bbrc.1995.2077
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
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