Effect of human leukemia inhibitory factor on in vitro development of IVF­derived bovine morulae and blastocysts

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Title
Effect of human leukemia inhibitory factor on in vitro development of IVF­derived bovine morulae and blastocysts
Author(s)
Yong Mahn Han; T. Mogoe; Kyung Kwang Lee; Y. Fukui
Bibliographic Citation
Theriogenology, vol. 44, pp. 507-516
Publication Year
1995
Abstract
This study was conducted to investigate whether human leukemia inhibitory factor (hLIF) improves the subsequent development of IVF-derived bovine morulae and blastocysts. To obtain IVF-derived bovine morulae, ova were matured and fertilized in vitro and cultured in 0.5 ml of synthetic oviduct fluid (SOF) medium supplemented with 10% human serum (HS) for 5 d at 39 °C under a gas atmosphere of 5% CO2, 5% O2, 90% N2. Morulae and early blastocysts at Day 5 of culture were cultured in 0.5 ml of SOF medium with or without 5000 U/ml recombinant hLIF for 2 or 3 d (2 groups). To investigate the effect of addition of hLIF on the subsequent development of morulae, SOF medium was supplemented with 8 mg/ml BSA instead of HS. To test whether hLIF affects the subsequent development of IVF-derived bovine blastocysts, only good blastocysts that developed from SOF medium with or without hLIF at Days 7 and 8 of culture were frozen by a conventinal slow freezing method and again cultured in SOF medium with or without the addition of hLIF for 3 d after thawing (4 groups). Survival of frozen-thawed bovine embryos was evaluated for re-expansion and hatching of blastocysts during 3 d of culture. There was no significant difference in the developmental rate of Day 5 embryos to blastocysts between those cultured with (47.8%) and without (47.6%) addition of hLIF. However, the addition of hLIF before freezing significantly increased the hatching rate of IVF-derived bovine morulae (P < 0.05), whereas addition of hLIF after thawing did not increase the subsequent development of blastocysts. These results suggest that hLIF added at the Day 5 morula stage may contribute to bovine embryonic development through the hatching process.
Keyword
cattlefreezinghLIFin vitro development
ISSN
0093-691X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0093-691X(95)00222-T
Type
Article
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1. Journal Articles > Journal Articles
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