Molecular cloning of GAF2, a Schizosaccharomyces pombe GATA factor, which has two zinc-finger sequences

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Title
Molecular cloning of GAF2, a Schizosaccharomyces pombe GATA factor, which has two zinc-finger sequences
Author(s)
Kwang Lae Hoe; Mi-Sun Won; Ook-Joon Yoo; Hyang Sook Yoo
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 39, no. 1, pp. 127-135
Publication Year
1996
Abstract
By low stringency screening of a λ-Shizosaccharomyces pombe genomic library, we have cloned a GATA factor homologous gene, gaf2+, within a 3.1-kb EcoRI fragment. The gaf2 ORF predicts a protein of Mr 61 kDa consisting of intronless 564 amino acids corresponding to 1,692 bp. Gaf2 has two zinc-fingers as Urbs1 of Ustilago maydis, whereas most of fungal GATA factors have only one zinc-finger. The separation between two zinc-fingers of Gaf2 is rather long. In addition to gaf2, the sequence analysis revealed a Val-tRNA gene in the 3′-flanking region of gaf2. Northern blot analysis indicated that the gaf2 gene is transcribed constitutively irrespective of the nitrogen source in a medium.
Keyword
S. pombeval-tRNAzinc fingerGATA factorlow stringency screening
ISSN
1039-9712
Publisher
Wiley
Type
Article
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
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