Cloning and characterization of cDNA for human adenylate kinase 2A

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Title
Cloning and characterization of cDNA for human adenylate kinase 2A
Author(s)
Yong Hee Lee; Jae Wha Kim; In Ae Lee; Ho Bum Kang; Yong Kyung Choe; Hee Gu Lee; Jong-Seok Lim; Hyo Joon KIm; Chankyu Park; In Seong Choe
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 39, no. 4, pp. 833-842
Publication Year
1996
Abstract
We have isolated and characterized a cDNA clone encoding human adenylate kinase 2A (AK2A) from cDNA libraries of fetal liver origin. The complete nucleotide sequence of the cloned 889 nucleotide cDNA fragment indicated that the deduced gene product of human AK2A is composed of 239 amino acids with a molecular weight of 26 kD. Comparison of these nucleotide and amino acid sequences with the corresponding sequences of bovine AK2A and rat AK2 revealed a high degree of conservation. It showed 91% and 98% homologies in DNA and amino acid sequences, respectively, with bovine AK2A throughout the full open reading frame. RNA blot analysis revealed that three species of mRNA were present with approximate sizes of 3.4, 2.1, and 1.0 kb. This gene was expressed in E. coli cells and the recombinant protein was enzymatically active.
Keyword
AK activitycDNAExpressionHuman adenylate kinase 2ARecombinant protein
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549600201931
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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