Overexpression and purification of human immunodeficiency virus type 1 env derived epitopes in Escherichia coli

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Title
Overexpression and purification of human immunodeficiency virus type 1 env derived epitopes in Escherichia coli
Author(s)
Mi Jin Sohn; Mi-Eun Lee; Hyo-Soon Park; Sang-Uk Nham; Young Ik Lee
Bibliographic Citation
Journal of Biotechnology, vol. 45, no. 3, pp. 211-216
Publication Year
1996
Abstract
In order to develop a reliable and inexpensive serodiagnostic method, a part of envelope gene of HIV-1, gp120' and gp41' (HIV-1 env a.a. 295-474 and a.a. 556-647) was cloned into a T7 expression vector (pET3d). The fusion protein (gp120'-gp41') was overexpressed in Escherichia coli, then purified to homogeneity by a simple gel filtration chromatography. Western blot analysis and enzyme-linked immunosorbent assay (ELISA) using the purified fusion protein showed a high sensitivity and a specificity for the detection of anti HIV-1 antibodies in testing human plasma. These results suggest that the expression scheme employing a direct expression vector and the rapid purification method are reliable and applicable for obtaining a large quantity of HIV-1 env protein for diagnoses of HIV-1 infections.
Keyword
direct expressionELISAenvelopeepitopeescherichia colifusion proteingp120gp41HIV-1T7 expression vector
ISSN
0168-1656
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0168-1656(95)00169-7
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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