Vinblastine determination measured by a sensitive ELISA inhibition assay = ELISA inhibition assay에 의한 Vinblastine의 측정

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Title
Vinblastine determination measured by a sensitive ELISA inhibition assay = ELISA inhibition assay에 의한 Vinblastine의 측정
Author(s)
Jae Wha Kim; Mi Young Han; Hee Gu Lee; Eun Young Song; Tai Wha Chung; Kyung Soo Nam; In Seong Choe
Bibliographic Citation
Korean Journal of Experimental & Biomedical Science, vol. 2, no. 1, pp. 121-126
Publication Year
1996
Abstract
Specific monoclonal antibodies(mAbs) against bis-indole alkaloids related to vinblastine were established to develop a simple and specific immunoassay system for the quantitation of Vinca alkaloids. Vinca alkaloids were extracted from tissue cultured cells of periwinkle plant (Vinca rosea L.). Spleen cells from Balb/c mice immunized with vinblastine-bovine serum albumin(VBL-BSA) conjugate as immunogen were fused with myeloma cells(Sp2/0-Ag.l4) in the presence of polyethylene glycol. In the preliminary experiments, 32 clones which highly reacted with VBL-BSA conjugate were selected by ELISA(Enzyme-linked immunosorbent assay). These clones were further analyzed by inhibition assay of ELISA. The results obtained with two typical monoclonal antibodies, KN-1 and KN-2, were described. KN-1 exhibited considerable reactivities with soluble dimeric bis-indole alkaloids, whereas no cross reacted with monomeric bis-indole alkaloids. However KN-2 showed cross reactivity with mono- and di-meric bis-indole alkaloids. Furthermore, KN-1 was applied to the immunoassay system for determining the VBL amounts of in vitro cultured cell extracts. This assay system could detect dimeric vinca alkaloid as low as 0.05 nM.
Keyword
VinblastineEnzyme-linked immunosorbent assay(ELISA)Inhibition assay of ELISA.
ISSN
I000-0011
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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