The inhibitory effects of Ampelocissus martinii Planch. extract on LPS-induced inflammation and oxidative stress in RAW 264.7 cells = RAW264.7 대식세포에서 Ampelocissus martinii Planch. 추출물의 LPS로 유도한 염증과 산화적 스트레스에 대한 저해 효과

Abstract

Chronic inflammation contribute to the development of Crohn’s disease and ulcerative colitis and cancer. This study focused on evaluating the anti-inflammatory and antioxidant effects of Ampelocissus martini Planch. extract (AME) in RAW264.7 cells. AME significantly suppressed lipopolysaccharide (LPS)-induced nitric oxide production and inducible nitric oxide synthase expression in RAW264.7 cells. AME also significantly suppressed LPS-induced tumor necrosis factor-α mRNA expression and phosphorylatioin of Jun N-terminal kinase. In addaition, AME significantly suppressed LPS-induced reactive oxygen species production in RAW264.7 cells. Also, AME had ABTS and DPPH radical scavenging capacity as much as ascorbic acid known as antioxidants. The results of study suggest that AME can be health functional food material with anti-inflammatory and antioxidant effects. 본 연구에서 AME는 RAW264.7 대식세포에서 LPS로 인해 유도된 JNK 1/2의 인산화를 억제하며 TNF-α mRNA 및 iNOS의 발현과 그에 따른 NO 생성을 억제하여 항염증 효과가 있음을 입증하였으며 또한 ROS 생성을 억제하고 자유 라디칼 소거능을 지녀 항산화 효과가 있음을 입증하였다. AME는 항염증 및 항산화 기능을 지닌 건강기능식품소재로 개발하는 데 좋은 정보가 될 것이라 생각한다.