Genomic heterogeneity in clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis

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Title
Genomic heterogeneity in clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex and M. fortuitum by pulsed-field gel electrophoresis
Author(s)
Jeong Ran Kim; Bong Seok Kang; Jung Hun Koh; J S Park; S J Kim; G H Bai; T H Chung; K S Nam; Yong Kyung Choe; In Seong Choe; Tai Wha Chung; Young Choon Lee; Chul Ho Kim
Bibliographic Citation
BMB Reports, vol. 29, no. 6, pp. 569-573
Publication Year
1996
Abstract
Clinical strains of Mycobacterium tuberculosis, M. terrae complex, M. gordonae, M. avium-intracellulae complex, and M. fortuitum from Korean patients were isolated and analyzed by comparing large restriction fragment (LRF) patterns produced by digestion of genomic DNA with infrequent-cutting endonucleases like AsnI and XbaI, and pulsed-field gel electrophoresis (PFGE). Three M. tuberculosis, two M. terrae complex, two M. gordonae, two M. avium-intracellulae complex, and two M. fortuitum strains were compared by using AsnI and XbaI, and this allowed easy visual separation of all epidemiologically unrelated strains. PFGE exhibits different DNA restriction patterns which are easy to compare. Genome size of the strains roughly ranged from 3020 to 3335 kb. The LRF patterns are useful for epidemiologic studies of tuberculosis with regard to drug resistance.
Keyword
genomemycobacteriapulsed-fielded gel electrophoresis
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Genome Editing Research Center > 1. Journal Articles
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