Determination of the three-dimensional structure of hordothionin-α by nuclear magnetic resonance

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Title
Determination of the three-dimensional structure of hordothionin-α by nuclear magnetic resonance
Author(s)
Kyou Hoon Han; Kyu Hwan Park; Hyun-Ju Yoo; Hoon Cha; Se WON Suh; F Thomas; Tae-Sung Moon; Seung Moak Kim
Bibliographic Citation
Biochemical Journal, vol. 313, no. 3, pp. 885-892
Publication Year
1996
Abstract
The high-resolution three-dimensional solution structure of the plant toxin hordothionin-α obtained from Korean barley was determined by using two-dimensional NMR techniques combined with distance geometry and restrained molecular dynamics. Experimentally derived restraints including 292 interproton distances from nuclear Overhauser effect measurements, 16 hydrogen bond restraints together with four disulphide bridge restraints were used as input to calculations of distance geometry and restrained molecular dynamics. Also included in the calculations were 36 ? and 17 χ1 torsion angles obtained from 3J(HNα) and 3J(αβ) coupling constants in double quantum filtered COSY and primitive exclusive COSY experiments, respectively. The overall protein fold is similar to crambin and purothionin-α1. Two α-helices running in opposite directions are found on the basis of 3J(HNα) and 3J(αβ) and deuterium exchange rates for backbone NH protons, and encompass residues 7-18 and 22-28. These two helices are connected by a turn and form a 'helix-turn-helix' motif. A short stretch of an anti-parallel β-sheet exists between residues 1-4 and 31-34. The two protein termini of hordothionin-α are 'well-anchored': the N-terminus of the protein is immobilized by this short β-sheet whereas the C-terminus is 'pasted' to the carbonyl group of Cys-4 by a very stable hydrogen bond, The average root-mean-square differences for the backbone and heavy atoms after the restrained molecular dynamics calculations are 0.62 and 1.16 ? respectively. These numbers represent a significant improvement over the corresponding values for the previous NMR structures of other thionins. The distance violation from the experimental interproton distances for the final structures is 0.14 ? for all atoms.
ISSN
0264-6021
Publisher
Portland Press Ltd
DOI
http://dx.doi.org/10.1042/bj3130885
Type
Article
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1. Journal Articles > Journal Articles
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