Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody(MabB23) specific for human plasma apolipoprotein B-100

Abstract

We have determined the nucleotide sequences encoding the heavy and light chains of the Fab fragment of murine monoclonal antibody MabB23 (γ2b,λ), which is specific for human plasma apolipoprotein B-100 of low-density lipoproteins. The sequence analyses revealed that the variable regions of the heavy and light chains are members of mouse heavy-chain subgroup I(B) and λ light-chain, respectively. A few unusual amino acids in the framework and constant regions of the heavy-chain were also noticed.