An enzyme-linked immunoassay for the levansucrase of Zymomonas mobilis using specific antibodies produced against the cloned enzyme

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Title
An enzyme-linked immunoassay for the levansucrase of Zymomonas mobilis using specific antibodies produced against the cloned enzyme
Author(s)
Ju Won Kwak; Young Gyo Seo; Ki Bang Song; Sang Ki Rhee
Bibliographic Citation
Biotechnology Techniques, vol. 10, no. 2, pp. 127-132
Publication Year
1996
Abstract
Levansucrase gene (levU) of Z. mobilis had been cloned and overexpressed in Escherichia coli (Song, K.-B. and Rhee, S.-K. (1994) Biotechnol. Lett. 16, 1305-1310). The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-linked immunosorbent assay (ELISA) amenable to immunochemical analysis of the levansucrase has been developed. The working range of levansucrase concentration in the developed ELISA was between 75 and 300 ng/ml. The assay was very sensitive to detect the levansucrase as little as 37 ng/ml.
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00765195
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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