Somatic embryogenesis and plant regeneration in cotyledonary explant cultures of Chinese cabbage

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Title
Somatic embryogenesis and plant regeneration in cotyledonary explant cultures of Chinese cabbage
Author(s)
Pil S Choi; Woong Y Soh; Jang Ryol Liu
Bibliographic Citation
Plant Cell Tissue and Organ Culture, vol. 44, pp. 253-256
Publication Year
1996
Abstract
Cotyledonary explants of Chinese cabbage were cultured on Murashige and Skoog's medium supplemented with various concentrations of 2,4- dichlorophenoxyacetic acid. Up to 20% of the cotyledonary explants produced somatic embryos with or without intervening callus production. Explants became more competent as the age of the source seedlings increased up to 8 days, but cotyledonary explants from 10-day-old seedlings were not responsive. Upon transfer to MS basal medium most of the somatic embryos developed into plantlets. Plantlets were transplanted to potting soil and grown to maturity in a phytotron. Among three cultivars used, only cotyledonary explants of 'Top Salad' were capable of producing somatic embryos.
Keyword
brassica campestris ssp. napus pekinensistissue culture
ISSN
0167-6857
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00048532
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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