Bestatin analogue from Streptomyces neyagawaensis SL-387

Abstract

A bestatin analogue, (2S,3R)-3-amino-2-hydroxy-4- phenylbutanoyl-L- valine (AHPA-Val), from the culture filtrate of Streptomyces neyagawaensis SL-387 was obtained in a chemically defined medium containing DL- 3-amino-3- phenylpropionic acid. AHPA-Val was 6 times (IC50=1.2 μg/ml) as strong as bestatin (IC50=7.0 μg/ml) against porcine kidney microsomal aminopeptidase N, and 4 times (5.6 μg/ml) as strong as bestatin (IC50= 20.7 μg/ml) against amino-peptidase N of human metastatic fibrosarcoma HT1080. To the best of our knowledge, this is the first report on the microbial production of AHPA-Val.