DC Field | Value | Language |
---|---|---|
dc.contributor.author | Dae Ook Kang | - |
dc.contributor.author | In Kyu Hwang | - |
dc.contributor.author | Bo Yeon Kim | - |
dc.contributor.author | Soon Cheol Ahn | - |
dc.contributor.author | Tae Ick Mheen | - |
dc.contributor.author | Jong Seog Ahn | - |
dc.contributor.author | Si Myung Byun | - |
dc.date.accessioned | 2017-04-19T08:45:28Z | - |
dc.date.available | 2017-04-19T08:45:28Z | - |
dc.date.issued | 1996 | - |
dc.identifier.issn | 1039-9712 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3753 | - |
dc.description.abstract | For the secretion of Bacillus stearothermophilus α-amylase from yeast, a recombinant plasmid pGAT17 was constructed by fusing B. stearothermophilus a -amylase structural gene in frame to the promoter and signal sequence of Saccharomyces diastaticus glucoamylase I gene (STA1). The secretion of the heterologous α-amylase from S. diastaticus transformed with pGAT17 was confirmed by the halo formation around colonies on selective starch agar medium. About 80% of the total α-amylase activity was detected in the extracellular culture medium. The secreted α-amylase was glycosylated and its molecular weight increased from 61 kDa to 75 kDa. The thermostability of the the glycosylated a -amylase was markedly enhanced, compared with that of the non-glycosylated enzyme from E. coli. | - |
dc.publisher | Wiley | - |
dc.title | Secretion of Bacillus α-amylase from yeast directed by glucoamylase I signal sequence of Saccharomyces diastaticus | - |
dc.title.alternative | Secretion of Bacillus α-amylase from yeast directed by glucoamylase I signal sequence of Saccharomyces diastaticus | - |
dc.type | Article | - |
dc.citation.title | Biochemistry and Molecular Biology International | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 190 | - |
dc.citation.startPage | 181 | - |
dc.citation.volume | 39 | - |
dc.contributor.affiliatedAuthor | Dae Ook Kang | - |
dc.contributor.affiliatedAuthor | Bo Yeon Kim | - |
dc.contributor.affiliatedAuthor | Soon Cheol Ahn | - |
dc.contributor.affiliatedAuthor | Tae Ick Mheen | - |
dc.contributor.affiliatedAuthor | Jong Seog Ahn | - |
dc.contributor.alternativeName | 강대욱 | - |
dc.contributor.alternativeName | 황인규 | - |
dc.contributor.alternativeName | 김보연 | - |
dc.contributor.alternativeName | 안순철 | - |
dc.contributor.alternativeName | 민태익 | - |
dc.contributor.alternativeName | 안종석 | - |
dc.contributor.alternativeName | 변시명 | - |
dc.identifier.bibliographicCitation | Biochemistry and Molecular Biology International, vol. 39, no. 1, pp. 181-190 | - |
dc.identifier.doi | 10.1080/15216549600201181 | - |
dc.description.journalClass | Y | - |
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