GERI-BP002-A, novel inhibitor of Acyl-CoA : cholesterol acyltransferase produced by Aspergillus fumigatus F93

Abstract

A new inhibitor of acyl-CoA : cholesterol acyltransfcrase (ACAT), designated GERI-BP002-A, was isolated from the culture broth of Aspergillus fumigalus F93 by acetone extraction, EtOAc extraction, SiO2 column chromatography and reverse phase HPLC. Spectroscopic analyses of the compound identified bis (2-hydroxy-3-tert-butyl-5-methylphenyl) methane as the structure and its molecular weight and formula to be 340 and C23H32O2, respectively. GERI-BP002-A inhibited ACAT activity by 50% at the concentration of 50 μM in an enzyme assay system using rat liver microsomes.