Xanthomonas campestris pv. campestris에 대한 Bacillus sp. B19-108의 저해효과 = Antibacterial activity of Bacillus sp. B19-108 against phytopathogen Xanthomonas campestris pv. Campestris

Abstract

Xanthomonas campestris pv.campestris (Xcc) is the causative agent of black rot, a disease that severely damages cruciferous plants. However, there are no commercially registered chemicals or biologics for the control of this disease. In this study, antibacterial activity against microorganisms isolated from soil was evaluated using Xanthomonas campestris, a strain that causes black rot. Among them, strains with excellent antibacterial activity and enzyme activity were selected and 16S rRNA sequencing was performed. As a result, it was identified as the genus Bacillus and named Bacillus sp. B19-108. In order to secure the maximum number of B19-108 bacteria, an optimal production medium composition was established, and a slightly acidic pH condition and a culture temperature condition of 36o C were established. After powder formulation of Bacillus sp. B19-108, the effect of inhibiting the occurrence of V-shaped yellow lesions, a symptom caused by Xcc infection, was investigated using Chinese cabbage. As a result of the experiment, it was confirmed that the range of V-shaped yellow lesions was reduced in the Bacillus sp. B19-108 treatment group compared to the Xcc alone treatment group. Therefore, it is considered that additional studies such as extended efficacy evaluation for cruciferous crops using Bacillus sp B19-108 strain and field tests in facility cultivation are needed.