Molecular cloning and induction of β-1,3-glucanase gene from Nicotiana glutinosa L.

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dc.contributor.authorHae Keun Yun-
dc.contributor.authorSo Young Yi-
dc.contributor.authorSung Uk Kim-
dc.contributor.authorKwang Hee Son-
dc.contributor.authorSeung Hun Yu-
dc.contributor.authorSong Hae Bok-
dc.contributor.authorDo Il Choi-
dc.description.abstractCloning and characterization of disease-response genes in plants could be an initial step toward understanding the complex disease resistance mechanism. To better understand the complex step, we isolated one of the pathogenesis-related proteins, β-1,3-glucanase, cDNA from a cDNA library of Nicotiana glutinosa showing systemic resistance. One clone (GN-3) was a partial cDNA of β-1,3-glucanase 800 bp in size with a 171 amino acid coding region. This clone had a 90% nucleotide homology with the β-1,3-glucanase gene of N. tabacum cv. BY4. A deduced amino acid sequence of GN-3 clones indicated a 91% identity with the β-1,3-glucanase of tobacco, 58% with that of Lycopersicon esculentum, and 51% with that of Glycine max. Northern blot analysis showed that expression of β-1,3-glucanase mRNAs was induced by TMV infection and salicylic acid treatment. In addition to that this gene was highly induced by CuSO4 and β-aminobutyric acid which are known as inducers of plant disease resistance. The possible role of this gene expression in relation to chemical-induced plant defense responses is discussed.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMolecular cloning and induction of β-1,3-glucanase gene from Nicotiana glutinosa L.-
dc.title.alternativeMolecular cloning and induction of β-1,3-glucanase gene from Nicotiana glutinosa L.-
dc.citation.titleMolecules and Cells-
dc.contributor.affiliatedAuthorHae Keun Yun-
dc.contributor.affiliatedAuthorSo Young Yi-
dc.contributor.affiliatedAuthorSung Uk Kim-
dc.contributor.affiliatedAuthorKwang Hee Son-
dc.contributor.affiliatedAuthorSong Hae Bok-
dc.contributor.affiliatedAuthorDo Il Choi-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 6, no. 4, pp. 422-428-
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Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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