Partial interfacial activation of Proteus vulgaris lipase overexpressed in Escherichia coli

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Title
Partial interfacial activation of Proteus vulgaris lipase overexpressed in Escherichia coli
Author(s)
Hyung Kwoun Kim; Young Seo Park; Hyoung Man Kim; Tae Kwang Oh
Bibliographic Citation
Bioscience Biotechnology and Biochemistry, vol. 60, no. 8, pp. 1365-1367
Publication Year
1996
Abstract
An expression plasmid carrying an alkaline lipase gene from Proteus vulgaris was constructed. The lipase content in Escherichia call cells harboring the expression plasmid reached about 22% of total soluble protein. The purified enzyme displayed a partial interfacial activation toward p- nitrophenyl butyrate (PNPB).
Keyword
Interfacial activationLidLipaseProteus vulgaris
ISSN
0916-8451
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1271/bbb.60.1365
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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