Optimal conditions for levan formation by an overexpressed recombinant levansucrase

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Title
Optimal conditions for levan formation by an overexpressed recombinant levansucrase
Author(s)
H Belghith; Ki Bang Song; Chul Ho Kim; Sang Ki Rhee
Bibliographic Citation
Biotechnology Letters, vol. 18, no. 4, pp. 467-472
Publication Year
1996
Abstract
A genetically modified levansucrase, which contained His-affinity tag in its C-terminal, was constructed by PCR reaction using two synthetic primers. This modified protein was produced up to 30% in total cell protein of E. coli, and was purified by a one-step affinity chromatography. The optimum pH for levan production was pH 5 and the optimum temperature was 0°C. The higher velocity of levan formation within shorter enzyme reaction times was achieved by increasing the levels of enzyme concentration. The optimal sucrose concentration for levan production was around 20%. Under these conditions, more than 50 g levan/l was produced.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00143472
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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