|dc.contributor.author||Seung Goo Lee||-|
|dc.contributor.author||Seung Pyo Hong||-|
|dc.contributor.author||Moon Hee Sung||-|
|dc.description.abstract||This study explores an enzymatic method for removing phenol from the wastewater created during the manufacture of phenolic resin. The enzyme used was a thermostable β-tyrosinase catalyzing the synthesis of L-tyrosine from phenol, pyruvate, and ammonia. As the reaction proceeds, L-tyrosine precipitates as insoluble aggregates because L-tyrosine is barely soluble in water. The enzymatic removal of phenol was effective at pH values ranging from 6.5-9.0 and temperatures below 70°C. The optimal concentration of each substrate was determined as 60 mM phenol, 0.1 M pyruvate, and 0.4 M ammonia. When the enzyme was used in an intact cell or acetone-dried cell state instead of the cell-free extract, the optimal concentration of phenol was increased up to 120 mM. By treating wastewater containing 100 mM phenol with acetone-dried cells at 37°C, we could reduce the concentration of phenol to 8 mM within 24h.||-|
|dc.title||Removal and bioconversion of phenol in wastewater by a thermostable β-tyrosinase||-|
|dc.title.alternative||Removal and bioconversion of phenol in wastewater by a thermostable β-tyrosinase||-|
|dc.citation.title||Enzyme and Microbial Technology||-|
|dc.contributor.affiliatedAuthor||Seung Goo Lee||-|
|dc.contributor.affiliatedAuthor||Seung Pyo Hong||-|
|dc.contributor.affiliatedAuthor||Moon Hee Sung||-|
|dc.identifier.bibliographicCitation||Enzyme and Microbial Technology, vol. 19, pp. 374-377||-|
|dc.subject.keyword||enzymatic removal of phenol||-|
|dc.subject.local||enzymatic removal of phenol||-|
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