Removal and bioconversion of phenol in wastewater by a thermostable β-tyrosinase

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dc.contributor.authorSeung Goo Lee-
dc.contributor.authorSeung Pyo Hong-
dc.contributor.authorMoon Hee Sung-
dc.date.accessioned2017-04-19T08:45:33Z-
dc.date.available2017-04-19T08:45:33Z-
dc.date.issued1996-
dc.identifier.issn0141-0229-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3797-
dc.description.abstractThis study explores an enzymatic method for removing phenol from the wastewater created during the manufacture of phenolic resin. The enzyme used was a thermostable β-tyrosinase catalyzing the synthesis of L-tyrosine from phenol, pyruvate, and ammonia. As the reaction proceeds, L-tyrosine precipitates as insoluble aggregates because L-tyrosine is barely soluble in water. The enzymatic removal of phenol was effective at pH values ranging from 6.5-9.0 and temperatures below 70°C. The optimal concentration of each substrate was determined as 60 mM phenol, 0.1 M pyruvate, and 0.4 M ammonia. When the enzyme was used in an intact cell or acetone-dried cell state instead of the cell-free extract, the optimal concentration of phenol was increased up to 120 mM. By treating wastewater containing 100 mM phenol with acetone-dried cells at 37°C, we could reduce the concentration of phenol to 8 mM within 24h.-
dc.publisherElsevier-
dc.titleRemoval and bioconversion of phenol in wastewater by a thermostable β-tyrosinase-
dc.title.alternativeRemoval and bioconversion of phenol in wastewater by a thermostable β-tyrosinase-
dc.typeArticle-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.number0-
dc.citation.endPage377-
dc.citation.startPage374-
dc.citation.volume19-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.affiliatedAuthorSeung Pyo Hong-
dc.contributor.affiliatedAuthorMoon Hee Sung-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName홍승표-
dc.contributor.alternativeName성문희-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, vol. 19, pp. 374-377-
dc.identifier.doi10.1016/S0141-0229(96)00001-4-
dc.subject.keywordenzymatic removal of phenol-
dc.subject.keywordthermostable β-tyrosinase-
dc.subject.keywordwastewater treatment-
dc.subject.localenzymatic removal of phenol-
dc.subject.localthermostable β-tyrosinase-
dc.subject.localwastewater treatment-
dc.subject.localWastewater treatment-
dc.description.journalClassY-
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Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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