Cloning and overexpression of thermostable D-hydantoinase from thermophile in E. coli and its application to the synthesis of optically active D-amino acids

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dc.contributor.authorDong Chul Lee-
dc.contributor.authorSeung Goo Lee-
dc.contributor.authorSeung Pyo Hong-
dc.contributor.authorMoon Hee Sung-
dc.contributor.authorHak Sung Kim-
dc.date.accessioned2017-04-19T08:45:33Z-
dc.date.available2017-04-19T08:45:33Z-
dc.date.issued1996-
dc.identifier.issn0077-8923-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3799-
dc.description.abstractWe cloned the thermostable D-hydantoinase gene from B. stearothermophilus SD-1 into E. coli. The cloned gene was constitutively expressed by its own promoter, and the enzyme was produced in its soluble form. The specific activity of the recombinant E. coli was 30 times higher than that of B. stearathermophilus SD-1. The cultivation conditions were investigated for the overproduction of the enzyme, and the temperature was found to affect the plasmid content and the expression level of the enzyme. Recombinant E. coli was cultivated in 30-L batch fermentation, the cell concentration reached 25 g-DCW/L, and the specific activity was about 20,000 units/g-DCW. D-Hydantoinase produced from the recombinant E. coli could be successfully applied to the synthesis of N-carbarnoyl-D-amino acid from the 5-monosubstituted hydantoin derivative.-
dc.publisherWiley-
dc.titleCloning and overexpression of thermostable D-hydantoinase from thermophile in E. coli and its application to the synthesis of optically active D-amino acids-
dc.title.alternativeCloning and overexpression of thermostable D-hydantoinase from thermophile in E. coli and its application to the synthesis of optically active D-amino acids-
dc.typeArticle-
dc.citation.titleAnnals of New York Academy of Sciences-
dc.citation.number0-
dc.citation.endPage405-
dc.citation.startPage401-
dc.citation.volume799-
dc.contributor.affiliatedAuthorDong Chul Lee-
dc.contributor.affiliatedAuthorSeung Goo Lee-
dc.contributor.affiliatedAuthorSeung Pyo Hong-
dc.contributor.affiliatedAuthorMoon Hee Sung-
dc.contributor.alternativeName이동철-
dc.contributor.alternativeName이승구-
dc.contributor.alternativeName홍승표-
dc.contributor.alternativeName성문희-
dc.contributor.alternativeName김학성-
dc.identifier.bibliographicCitationAnnals of New York Academy of Sciences, vol. 799, pp. 401-405-
dc.identifier.doi10.1111/j.1749-6632.1996.tb33232.x-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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