Effects of reaction additives on enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine = 3,4-dihydroxyphenyl-L-alanine의 효소적 생산에 대한 반응첨가물의 영향

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Title
Effects of reaction additives on enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine = 3,4-dihydroxyphenyl-L-alanine의 효소적 생산에 대한 반응첨가물의 영향
Author(s)
Seung Goo Lee; Hyeon Su Ro; Seung Pyo Hong; Moon Hee Sung
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 24, no. 2, pp. 222-226
Publication Year
1996
Abstract
The enzymatic synthesis of 3,4-dihydroxyphenyl-L-alanine (L-DOPA) was examined for the effects of the reaction additives such as sodium borate, alcohol, and organic solvents. The enzyme used was tyrosine phenol-lyase of Citrobacter fretindii KCTC 2006 produced in Escherichia coli. The amounts of tyrosine phenol-lyase and pyridoxal-5-phosphate were optimized to 2.0 units/ml and 0.1 mM, respectively, for the synthetic reaction. Sodium borate, a substance that forms a complex with pyrocatechol, reduced the enzyme deactivation by pyrocatechol although it seriously inhibited the enzyme activity. Among the organic solvents tested, dimethylsulfoxide, dimethylformamide, and alcohol increased the productivity of the L-DOPA synthesis. In a reaction system with 5% methanol, L-DOPA concentration increased up to 210 mM after 24 hours, and 77.1% of which was separated as precipitates. The L-DOPA was purified to 99.96% by solubilizing and recrystallyzing the precipitates.
Keyword
L-DOPAorganic solventpyridoxal-5-phosphatesodium boratetyrosine phenol-lyase
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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