Analysis of glucosinolate and phenylpropanoid contents in four different cultivars of radish sprouts

Abstract

Radish (Raphanus sativus L.) is a prominent root vegetable cultivated throughout the world because of its nutritional and bioactive components. This study is conducted for growth performance and phytochemical investigation of four different cultivars of radish sprouts i.e., white, yellow, red, and black. Growth parameters such as shoot length, root length, and fresh weight were recorded and the results showed that the red cultivar had the highest values in all aspects, showing the highest shoot length (5.72 cm), root length (12.1 cm), and fresh weight (283.7 mg). In the black cultivar, these parameters exhibited the lowest growth. Individual glucosinolates (GSLs) and phenylpropanoid compounds were also analyzed from all the cultivars. The analysis of 10-day-old radish sprouts revealed the presence of 7 glucosinolates and 10 phenylpropanoid compounds. The individual and total content of all the compounds varied significantly within cultivars. The highest amount of total glucosinolates (87.00 μmol/g DW) was detected in red whereas the lowest total was found in the yellow cultivar (60.78 μmol/g DW). The variation of glucoraphanin content among the cultivars varied largely than any other glucosinolate showing much more in the red and white cultivars. The accumulation of glucoraphasatin content was much higher irrespective of cultivars. As for phenylpropanoids, the red cultivar exhibited the greatest amounts of chlorogenic acid (56.34 μg/g DW) and benzoic acid (42.35 μg/g DW). Among all cultivars, the white cultivar had the lowest phenylpropanoid profile, especially in terms of benzoic acid. This study reveals wide variation in growth and phytochemicals among the radish cultivars cultured on soil pots the positive traits of the red cultivar showed enhanced yield and nutraceutical compounds.