Enzymatic conversion of glutaryl 7-aminocephalosporanic acid to 7-aminocephalosporanic acid with an immobilized glutaryl 7-aminocephalosporanic acid acylase

Abstract

Ghitaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. SY-77-1 was immobilized with oxiran acrylic beads for the production of 7-aminocephalosporanic acid (7-ACA) from glutaryl 7-aminocephalosporanic acid (GL 7-ACA). The immobilized enzyme maintained its activity at a constant level for 7 days, but lost 30% of its activity after 20 days. Optimal reaction conditions for the synthesis of 7-ACA were found to be 30°C and pH 8.0 using the immobilized enzyme. For the economic production of 7-ACA, substrate and enzyme concentrations were optimized to 60 mM and 0.5 g wet weight per 10 ml of reaction volume, respectively. Under optimized conditions, 50 mM 7-ACA was produced from 60 mM GL 7-ACA within 8 h, resulting in a conversion yield of 83%.