DC Field | Value | Language |
---|---|---|
dc.contributor.author | Won-Bae Kim | - |
dc.contributor.author | Jung-Keug Park | - |
dc.contributor.author | Weon Sang Choi | - |
dc.contributor.author | Soo-Ok Kim | - |
dc.contributor.author | Ong-Ju Chung | - |
dc.contributor.author | Hyun-Su Kim | - |
dc.contributor.author | Ik Hwan Kim | - |
dc.date.accessioned | 2017-04-19T08:45:36Z | - |
dc.date.available | 2017-04-19T08:45:36Z | - |
dc.date.issued | 1996 | - |
dc.identifier.issn | 1225-7117 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3819 | - |
dc.description.abstract | Attenuated varicella-zoster virus (VZV) was propagated in human lung fibroblast (HLF) cells. Among media tested in this work,DMEM was the best medium for the growth of HLF cells. Because HLF was a normal finite cell line, cell growth rate was dependent on the age of HLF cells. When the population doubling level (PDL) was higher than 46, apoptosis of HLF cells started and cell growth rate decreased. The optimum temperature for the cell growth and virus propagation in the T-flask culture was 37°C. In a microcarrier culture system in which Cytodex-3 was used for the VZV propagation in spinner vessels, the yield of plaque forming cells was lower than that in the T-flask culture. The relatively high shear environment near microcarriers was thought to cause the low yield of VZV in the microcarrier culture system. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Production of varicella-zoster virus using human lung fibroblast cells as host cells = 인체 폐섬유아세포 배양에 의한 수두바이러스의 생산 | - |
dc.title.alternative | Production of varicella-zoster virus using human lung fibroblast cells as host cells | - |
dc.type | Article | - |
dc.citation.title | Korean Journal of Biotechnology and Bioengineering | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 261 | - |
dc.citation.startPage | 254 | - |
dc.citation.volume | 11 | - |
dc.contributor.affiliatedAuthor | Ik Hwan Kim | - |
dc.contributor.alternativeName | 김원배 | - |
dc.contributor.alternativeName | 박정극 | - |
dc.contributor.alternativeName | 최원상 | - |
dc.contributor.alternativeName | 김수옥 | - |
dc.contributor.alternativeName | 정용주 | - |
dc.contributor.alternativeName | 김현수 | - |
dc.contributor.alternativeName | 김익환 | - |
dc.identifier.bibliographicCitation | Korean Journal of Biotechnology and Bioengineering, vol. 11, no. 2, pp. 254-261 | - |
dc.description.journalClass | N | - |
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