Description of Olsenella kribbiana sp. nov., an anaerobic bacterium isolated from pig faeces

Abstract

A short-rod-shaped, obligate anaerobic, Gram-stain-positive bacterium isolated from the faeces of a pig was designated as the strain YH-ols2221T. The analysis of the 16S rRNA gene sequence revealed that the isolate was most closely related to Olsenella phocaeensis DSM 103159T, Olsenella urininfantis KCTC 25755T and Olsenella absiana KCTC 25800T with 96.6-96.7% similarities. The multi-locus sequence tree revealed that the isolate formed a cluster adjacent to O. absiana KCTC 25800T. The average nucleotide identity and the digital DNA-DNA hybridization values for the isolate and the most closely related strains were 71.7-74.1% and 19.9-23.0%, respectively. The main fatty acids identified were C14 : 0, C16 : 0 DMA and C18 : 0 ω9c DMA. The cell wall contained the meso-diaminopimelic acid-based peptidoglycan. The end products of the fermentation were lactic acid and acetic acid. Given the chemotaxonomic, phenotypic and phylogenetic properties, YH-ols2221T (=KCTC 25801T=NBRC 116679T) represents a novel taxon. The name Olsenella kribbiana sp. nov. was proposed.