Down-regulation of protein kinase C in murine splenocytes : a potential mechanism for 2-acetylaminofluorene-mediated immunosuppression

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Title
Down-regulation of protein kinase C in murine splenocytes : a potential mechanism for 2-acetylaminofluorene-mediated immunosuppression
Author(s)
Michael Lee; Hwan Mook Kim; Kyu-Hwan Yang
Bibliographic Citation
Cancer Letters, vol. 101, pp. 53-57
Publication Year
1996
Abstract
2-Acetylaminofluorene (AAF), an arylamide carcinogen, inhibited in a dose dependent manner mouse spleen cell proliferation in response to lipopolysaccharide (LPS). The objective of the present studies was to investigate the effects of AAF on protein kinase C (PKC) activation, an enzyme required for LPS-induced splenocytes proliferation. After treatment with 50μM AAF for 18 h, PKC activity in the cytosolic fraction decreased by 50% from the control level, and splenocytes lost 30% of total PKC activity. Furthermore, as determined by the electrophoretic mobility shift assay, AAF inhibited the binding activity of the transcription factor complex, NF-κB, whose LPS-mediated induction is dependent on PKC activation in murine splenocytes. These results strongly suggest that LPS-mediated signaling in spleen cells is interrupted by AAF early in the signal transduction pathway, at a point proximal to the activation of PKC.
Keyword
2-acetylaminofluoreneImmunosuppressionProtein kinase C
ISSN
0304-3835
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0304-3835(96)04113-4
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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