Rapid determination of in vivo and in vitro antibody responses by suspension hemolytic assay

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Title
Rapid determination of in vivo and in vitro antibody responses by suspension hemolytic assay
Author(s)
Sang Bae Han; Goo Taeg Oh; Yeo-Pyo Yun; Byoung Gil Mheen; Byung Hwa Hyun; Hwan Mook Kim
Bibliographic Citation
Journal of Pharmacological and Toxicological Methods, vol. 36, pp. 33-40
Publication Year
1996
Abstract
A suspension hemolytic (SH) assay newly modified in this laboratory was applied to the measurement of primary IgM antibody responses, such as T-dependent, T-independent, and polyclonal B cell responses in in vivo and in vitro assays. All results of the SH assay were equal to those of the conventional plaque-forming cell (PFC) assay. With the SH assay in in vitro assays, we could separately measure the amount of the IgM that had been produced during the whole immunization period and measure the IgM newly produced during the shorter time of the assay. The immunomodulations by dioxin and glycopeptide were accurately evaluated by the SH and the PFC assay. Our study suggested that the SH assay modified in this laboratory was more advantageous because of the simplicity of the assay and better analysis of data than the PFC assay, and could be used as the primary method in studying the effects of pharmaceutical and toxic agents on humoral immune functions.
Keyword
Antibody responsesDioxinFCSGlycopeptidePFC assaySH assay
ISSN
1056-8719
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/1056-8719(96)00070-6
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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