Antifungal activities of peptides with the sequence 10-17 of magainin 2 at the N-termini against Aspergillus fumigatus

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Title
Antifungal activities of peptides with the sequence 10-17 of magainin 2 at the N-termini against Aspergillus fumigatus
Author(s)
Myung Kyu Lee; Dong Gun Lee; Song Yub Shin; Sung Gu Lee; Joo Hyun Kang; Kyung Soo Hahm
Bibliographic Citation
Journal of Microbiology, vol. 34, no. 3, pp. 274-278
Publication Year
1996
Abstract
Two peptides, MA-inv and MA-ME, with the sequence 10-17 of magainin 2 at their N-termini were designed and synthesized. The peptides had higher antifungal activities against Aspergillus fumigatus without hemolytic activities. The minimal inhibition concentratory (MIC) values of both peptides against A. fumigatus were 5 μg/ml, whereas those of the native peptides, magainin 2 and melittin, were 10 μg/ml. At 3 μg/ml, MA-inv and MA-ME inhibited the mycelium growth of A. fumigatus by 94.6% and 97.3%, respectively, whereas magainin 2 and melittin inhibited by 62.2% and 32.4%, respectively. MA-inv showed up to 80% inhibition of (1,3)-β-D-glucan synthase activity of A. fumigatus. The peptides also showed antifungal activities for other fungi of Aspergillus sp. However, the antibiotic activities of MA-ME against Escherichia coli, Bacillus subtilis and Fusarium oxysporum were more effective than those of MA-inv, suggesting that the C-terminal sequences of MA-inv and MA-ME may also influence their antibiotic activities. These results suggest that the N-terminal sequence of the designed peptides, KKFGKAFV, is important for their antifungal activities against A. fumigatus and their C-terminal sequences are related to the organism selectivity.
Keyword
Synthetic peptideAntifungal activityAspergillus fumigatusGlucan synthaseMagainin 2 derived peptide
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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