Decreased chimeric antibody productivity of KR12H-1 transfectoma during long-term culture results from decreased antibody gene copy number

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Title
Decreased chimeric antibody productivity of KR12H-1 transfectoma during long-term culture results from decreased antibody gene copy number
Author(s)
Jae Hoon Kim; Sung Won Bae; Hyo Jeong Hong; Gyun Min Lee
Bibliographic Citation
Biotechnology and Bioengineering, vol. 51, pp. 479-487
Publication Year
1996
Abstract
The stability of KR12H-1 transfectoma in regard to chimeric antibody production was examined during long-term, repeated batch culture without selection pressure using antibiotics. Both serum-supplemented and serum-free media were used. Regardless of the medium used, the specific antibody productivity (q(Ab)) of transfectoma decreased by 60% to 88% during 70-day culture. This loss of antibody productivity was not due mainly to the appearance of a nonproducing population (NP) of transfectoma. The percentage of a producing population (P), which was monitored by the limiting dilution method, remained over 90% until the end of culture, indicating that the q(Ab) of P decreased during the culture. Flow cytometric data also showed the increase of cell population with low fluorescence intensity during culture, indicating that the intracellular antibody content of P decreased. The subclones of P obtained at the end of long-term culture were further characterized. Compared with the q(Ab) of P at the beginning of long-term culture, the q(Ab) of most P subclones was significantly low, confirming that the loss of antibody productivity was due mainly to the decreased q(Ab) of P during long-term culture. The decreased antibody gene copy number of P subclones was found to be partly responsible for the decreased q(Ab) of P during long-term culture.
Keyword
chimeric antibodySouthern blotstabilitytransfectoma
ISSN
0006-3592
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/(SICI)1097-0290(19960820)51:4<479::AID-BIT11>3.0.CO;2-D
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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