Novel polyethylene glycol/levan aqueous two-phase system for protein partitioning

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Title
Novel polyethylene glycol/levan aqueous two-phase system for protein partitioning
Author(s)
Bong Hyun Chung; Won Kyung Kim; Ki Bang Song; Chul Ho Kim; Sang Ki Rhee
Bibliographic Citation
Biotechnology Techniques, vol. 11, no. 5, pp. 327-329
Publication Year
1997
Abstract
A novel aqueous two-phase system was made by mixing PEG (polyethylene glycol) and levan. The partitioning experiments were performed with six model proteins: horse heart cytochrome c, horse hemoglobin, horse heart myoglobin, hen egg albumin, bovine serum albumin and hen egg lysozyme. The addition of NaCl increased the partition coefficient of lysozyme but decreased that of albumin. The partition coefficients were sensitive to pH, increasing in the pH range of 6 to 8 for five of the proteins tested and decreasing slightly for lysozyme.
ISSN
0951-208X
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1018475529976
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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