DC Field | Value | Language |
---|---|---|
dc.contributor.author | Y K Kim | - |
dc.contributor.author | Bong Hyun Chung | - |
dc.contributor.author | S Yoon | - |
dc.contributor.author | Kyung Kwang Lee | - |
dc.contributor.author | B Lonnerdal | - |
dc.contributor.author | Dae Yeul Yu | - |
dc.date.accessioned | 2017-04-19T08:53:43Z | - |
dc.date.available | 2017-04-19T08:53:43Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0951-208X | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3892 | - |
dc.description.abstract | Human αs1-casein was expressed efficiently in Escherichia coli. The overproduced recombinant human a αs1-casein was about 25% of the total cell protein. Two different vectors were constructed to express Met-αs1-casein and Met-αs1-casein with a His-affinity tag at the C-terminus. Recombinant Met-s1-casein with a His-affinity tag was purified to homogeneity using Ni-nitrilotriacetic acid resin. N-terminal sequence of the first 10 amino acid residues of this purified protein was identical to that of mature human αs1-casein with an extra methionine residue at the N-terminus. | - |
dc.publisher | Springer | ko |
dc.title | High-level expression of human alphaS1-casein in Escherichia coli | - |
dc.title.alternative | High-level expression of human alphaS1-casein in Escherichia coli | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Techniques | - |
dc.citation.number | 9 | - |
dc.citation.endPage | 678 | - |
dc.citation.startPage | 675 | - |
dc.citation.volume | 11 | - |
dc.contributor.affiliatedAuthor | Bong Hyun Chung | - |
dc.contributor.affiliatedAuthor | Kyung Kwang Lee | - |
dc.contributor.affiliatedAuthor | Dae Yeul Yu | - |
dc.contributor.alternativeName | 김 | - |
dc.contributor.alternativeName | 정봉현 | - |
dc.contributor.alternativeName | 윤 | - |
dc.contributor.alternativeName | 이경광 | - |
dc.contributor.alternativeName | Lonnerdal | - |
dc.contributor.alternativeName | 유대열 | - |
dc.identifier.bibliographicCitation | Biotechnology Techniques, vol. 11, no. 9, pp. 675-678 | - |
dc.identifier.doi | 10.1023/A:1018463411263 | - |
dc.description.journalClass | N | - |
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.