Expression of a DNA sequence encoding for the pre-S2 region of hepatitis B virus in E. coli

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorYoung Chul Choi-
dc.contributor.authorJi Young Kim-
dc.contributor.authorMyeong Hee Yu-
dc.contributor.authorMoon Hi Han-
dc.date.accessioned2017-04-19T08:53:45Z-
dc.date.available2017-04-19T08:53:45Z-
dc.date.issued1986-
dc.identifier.issn0368-4881-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3903-
dc.description.abstractA DNA sequence involving pre-S2 region of Hepatitis B virus (HBV) was inserted between ompF and lacZ gene of the plasmid pORFl to give a recombinant plasmid pHBSC20. In the plasmid pHBSC20 harboring E. coli TK1046 cells, a “tribrid” protein with the translation product of the insert sandwiched between OmpF and -galactosidase was produced. The fusion protein was detected by SDS-polyacrylamide gel electrophoresis and the antigenicity of the pre-S2 region was determined by immunoblot analysis. The amount of the fusion protein was estimated as much as 1-5% of the total bacterial protein by densitometric scanning of the Coomassie blue-stained gel. Thus, bacteria transformed with pHBSC20 can provide abundant source of pre-S2- p -galactosidase fusion protein in which production of HBV vaccine. the pre-S2 region may be useful for the-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression of a DNA sequence encoding for the pre-S2 region of hepatitis B virus in E. coli-
dc.title.alternativeExpression of a DNA sequence encoding for the pre-S2 region of hepatitis B virus in E. coli-
dc.typeArticle-
dc.citation.titleKorean Biochemical Journal-
dc.citation.number4-
dc.citation.endPage390-
dc.citation.startPage383-
dc.citation.volume19-
dc.contributor.affiliatedAuthorMyeong Hee Yu-
dc.contributor.affiliatedAuthorMoon Hi Han-
dc.contributor.alternativeName최영철-
dc.contributor.alternativeName김지영-
dc.contributor.alternativeName유명희-
dc.contributor.alternativeName한문희-
dc.identifier.bibliographicCitationKorean Biochemical Journal, vol. 19, no. 4, pp. 383-390-
dc.description.journalClassN-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.