Expression of hepatitis B virus surface antigen gene in E. coli

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Title
Expression of hepatitis B virus surface antigen gene in E. coli
Author(s)
Young Chul Choi; Ji Young Kim; Myeong Hee Yu; Moon Hi Han
Bibliographic Citation
Korean Biochemical Journal, vol. 19, no. 4, pp. 377-382
Publication Year
1986
Abstract
Three different HBsAg sequences encoding for S region, pre-S2 plus S region, and pre-S2 fused with a portion of -galactosidase have been expressed in E. coli under the control of lac promoter. When the S region containing HBsAg genes were expressed constitutively in E. coli HB101, the growth inhibition of recombinant cells was observed. However, the constitutive expression of pre-S2 fused with a-peptide (N-terminal portion of 현 -galactosidase) was not detrimental to the E. coli HB101 cells. The antigenicity of the hybrid pre-S2 protein seems to be conformationindependent: treatment of 2-mercaptoethanol or 2-mercaptoethanol/SDS did not abol- ish the antigenicity.
ISSN
0368-4881
Publisher
Korea Soc-Assoc-Inst
Type
Article
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1. Journal Articles > Journal Articles
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