Dual hollow fiber membrane bioreactor for whole cell enzyme immobilization of Streptomyces griseus with glucose isomerase activity

Abstract

A new process using the dual hollow fiber bioreactor (DHFBR) system for whole cell enzyme immobilization was developed. This method allows Streptomyces griseus with glucose isomerase activity to proliferate in DHFBR to a desired density and convert glucose to fructose with high productivity. After 6 days the dry cell mass amounted to 140 g/l based on the space volume available for cell growth. The volumetric productivity of fructose by DHFBR was 22.5 g/l·h (based on 34% glucose conversion and the inner silicone tube volume), which correspond to a 12-fold increase over that of the batch method (1.8 g/l·h, based on 44% glucose conversion).