Effects of inoculation with selected Rhizobium japonicum on the yield, nodule formation and nitrogen fixing activity of soybean (Glycine max) = 선발대두근류균의 접종의 대두근류형성 질소고정활성 및 수량에 미치는 영향

Abstract

Apical meristem culture was used for high frequency embryogenic callus induction of sweet potato (Ipomoea batatas (L.) Lam; cv. White Star). Shoot apical meristem dome explants produced embryogenic callus on agar-solidified nutrient medium containing 0.5？3.0 mg/1 2, -4-dichlorophenoxyacetic acid (2,4？D). The frequency of embryogenic callus fonnation was over 90%. However, the frequency declined sharply as the number of leaf primordia pairs attached to the explant increased. The induced callus gave rise to numerous somatic embryos. U^on transfer onto medium containing 0.02 mg/1 abscisic acid (ABA) and 0.02 mg/1 N - (2-isopentenyl) adenine (2iP), embryos converted into plantlets.