Generation and characterization of induced pluripotent stem cell derived from cynomolgus monkey (cmESF-iPS-C4)

Abstract

Cynomolgus monkey iPSCs were generated from ear fibroblasts, offering a non-invasive and accessible cell source for autologous therapies and preclinical research. These iPSCs demonstrated robust pluripotency and differentiation potential, successfully giving rise to functional macrophages. Validation confirmed chromosomal stability, transgene clearance, and mycoplasma-free status. While this study focuses on establishing a reliable iPSC source, these cells hold promise for generating functional cell types for potential autologous applications in primate models. This platform provides a valuable foundation for advancing regenerative medicine and precision immunotherapy, bridging the gap between in vitro research and future translational studies.