Construction of a Clostridium acetobutylicum - Escherichia coli shuttle vector

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dc.contributor.authorKi Hong Yoon-
dc.contributor.authorJung Kee Lee-
dc.contributor.authorByung Hong Kim-
dc.date.accessioned2017-04-19T08:53:47Z-
dc.date.available2017-04-19T08:53:47Z-
dc.date.issued1991-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3924-
dc.description.abstractA 4.1-kb cryptic plasmid, designated pCA134, has been isolated fromClostridium species. In order to develop a vector suitable for transforming saccharolytic clostridia three hybrid plasmids were constructed by inserting pCA134 into pHV32 withEcoRI, orBglII andBamHI. The newly constructed plasmids were propagated inEscherichiacoli and were used to transformBacillussubtilis andClostridiumacetobutylicum. One of them, pCAB32 (10.1 kb), which contains chloramphenicol acetyltransferase gene and an origin of replication derived from pCA134 was introduced intoB.subtilis andC.acetobutylicum as well asE.coli.-
dc.publisherSpringer-
dc.titleConstruction of a Clostridium acetobutylicum - Escherichia coli shuttle vector-
dc.title.alternativeConstruction of a Clostridium acetobutylicum - Escherichia coli shuttle vector-
dc.typeArticle-
dc.citation.titleBiotechnology Letters-
dc.citation.number1-
dc.citation.endPage6-
dc.citation.startPage1-
dc.citation.volume13-
dc.contributor.affiliatedAuthorKi Hong Yoon-
dc.contributor.affiliatedAuthorJung Kee Lee-
dc.contributor.alternativeName윤기홍-
dc.contributor.alternativeName이정기-
dc.contributor.alternativeName김병홍-
dc.identifier.bibliographicCitationBiotechnology Letters, vol. 13, no. 1, pp. 1-6-
dc.identifier.doi10.1007/BF01033507-
dc.subject.keywordarticle-
dc.subject.keywordclostridium acetobutylicum-
dc.subject.keywordescherichia coli-
dc.subject.keywordnonhuman-
dc.subject.keywordrecombinant plasmid-
dc.subject.keywordClostridium acetobutylicum-
dc.subject.keywordEscherichia coli-
dc.subject.localarticle-
dc.subject.localClostridium acetobutylicum-
dc.subject.localclostridium acetobutylicum-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localE.coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.subject.localnonhuman-
dc.subject.localrecombinant plasmid-
dc.subject.localClostridium acetobutylicum-
dc.subject.localclostridium acetobutylicum-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localE.coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.description.journalClassY-
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