The retinoblastoma gene product regulates Sp1-mediated transcription

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Title
The retinoblastoma gene product regulates Sp1-mediated transcription
Author(s)
Seong-Jin Kim; U S Onwuta; Young Ik Lee; R Li; M R Botchan; P D Robbins
Bibliographic Citation
Molecular and Cellular Biology, vol. 12, no. 6, pp. 2455-2463
Publication Year
1992
Abstract
We have demonstrated that the retinoblastoma gene product (Rb) can positively regulate transcription from the fourth promoter of the insulinlike growth factor II gene. Two copies of a motif (the retinoblastoma control element) similar to that found in the human c-fos, transforming growth factor β1, and c-myc promoters are responsible for conferring Rb regulation to the fourth promoter of the insulinlike growth factor II gene. We have shown that the transcription factor Sp1 can bind to and stimulate transcription from the retinoblastoma control element motif. Moreover, by using a GAL4-Sp1 fusion protein, we have directly demonstrated that Rb positively regulates Sp1 transcriptional activity in vivo. These results indicate that Rb can function as a positive regulator of transcription and that Sp1 is one potential target, either directly or indirectly, for transcriptional regulation by Rb.
ISSN
0270-7306
Publisher
Amer Soc Microb
DOI
http://dx.doi.org/10.1128/MCB.12.6.2455
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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