Sex determination in single mouse blastomeres by polymerase chain reaction

Cited 23 time in scopus
Metadata Downloads
Title
Sex determination in single mouse blastomeres by polymerase chain reaction
Author(s)
Yong Mahn Han; Ook Joon Yoo; Kyung Kwang Lee
Bibliographic Citation
Journal of Assisted Reproduction and Genetics, vol. 10, no. 2, pp. 151-156
Publication Year
1993
Abstract
In sex determination of mammalian preimplantation embryos, viability of biopsied embryos and accuracy of sexing are together important. In consideration of this point, single blastomeres were mechanically isolated from mouse embryos using a micromanipulator and then sexed by polymerase chain reaction (PCR) using mouse Y chromosome-specific primers. All of 260 embryos biopsied at the four-cell and morula stage survived. Developmental rate of the embryos to normal blastocysts was 93 and 94%, respectively. Sex determination of single blastomeres was performed by amplification of a mouse Y chromosome-specific DNA sequence using PCR technique. The ratio of male to female embryos was 53 and 47%, respectively. The sex-determined embryos were transferred to the uteri of pseudopregnant recipients to test the consistency of the assay system. The sex of 27 of 29 mice developed from male and female embryos agreed with the predicted sex. The method developed for embryo biopsy and sexing could be used for diagnosis of defective genes at the stage of the preimplantation embryos of human and other domestic animals.
Keyword
embryo biopsypolymerase chain reactionsexing
ISSN
1058-0468
Publisher
Springer
DOI
http://dx.doi.org/10.1007/bf01207739
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.