DC Field | Value | Language |
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dc.contributor.author | Man-Jong Kang | - |
dc.contributor.author | Yong Mahn Han | - |
dc.contributor.author | Chul Sang Lee | - |
dc.contributor.author | Sang-Tae Shin | - |
dc.contributor.author | Kyung Kwang Lee | - |
dc.date.accessioned | 2017-04-19T08:53:57Z | - |
dc.date.available | 2017-04-19T08:53:57Z | - |
dc.date.issued | 1994 | - |
dc.identifier.issn | 1058-0468 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/3990 | - |
dc.description.abstract | Purpose: To clarify the developmental capacity of frozen two-cell blastomeres, we investigated in vivo and in vitro viabilities of blastomeres that were frozen ultrarapidly after separation from two-cell mouse embryos. Two-cell embryos obtained from superovulated F1 hybrid females were denuded by treatment with 0.5% pronase solution and then induced to separate into two single blastomeres by gentle pipetting. The blastomeres were cryopreserved by an ultrarapid freezing method. Results: The preimplantation developmental rate of two-cell embryos frozen in 3.0 M DMSO was significantly higher than the rate of those frozen in 15 and 4.5 M DMSO (at least P < 0.05). The in vitro developmental rate of the ultrarapidly frozen-thawed blastomeres separated from two-cell embryos (75.0%) was similar to that of nonfrozen blastomeres (76.0%). When eight pairs of blastocysts that developed from frozen two-cell mouse blastomeres were transferred to pregnant ICR recipients on Day 3, four live singletons were born. Conclusion: Thus, the results indicate that two-cell mouse blastomeres can be frozen by the ultrarapid freezing method. | - |
dc.publisher | Springer | - |
dc.title | Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method | - |
dc.title.alternative | Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method | - |
dc.type | Article | - |
dc.citation.title | Journal of Assisted Reproduction and Genetics | - |
dc.citation.number | 8 | - |
dc.citation.endPage | 413 | - |
dc.citation.startPage | 409 | - |
dc.citation.volume | 11 | - |
dc.contributor.affiliatedAuthor | Man-Jong Kang | - |
dc.contributor.affiliatedAuthor | Yong Mahn Han | - |
dc.contributor.affiliatedAuthor | Chul Sang Lee | - |
dc.contributor.affiliatedAuthor | Kyung Kwang Lee | - |
dc.contributor.alternativeName | 강만종 | - |
dc.contributor.alternativeName | 한용만 | - |
dc.contributor.alternativeName | 이철상 | - |
dc.contributor.alternativeName | 신상태 | - |
dc.contributor.alternativeName | 이경광 | - |
dc.identifier.bibliographicCitation | Journal of Assisted Reproduction and Genetics, vol. 11, no. 8, pp. 409-413 | - |
dc.identifier.doi | 10.1007/bf02211728 | - |
dc.subject.keyword | full-term development | - |
dc.subject.keyword | two-cell blastomeres | - |
dc.subject.keyword | ultrarapid freezing | - |
dc.subject.local | full-term development | - |
dc.subject.local | two-cell blastomeres | - |
dc.subject.local | ultrarapid freezing | - |
dc.description.journalClass | Y | - |
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