Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method

Cited 5 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorMan-Jong Kang-
dc.contributor.authorYong Mahn Han-
dc.contributor.authorChul Sang Lee-
dc.contributor.authorSang-Tae Shin-
dc.contributor.authorKyung Kwang Lee-
dc.date.accessioned2017-04-19T08:53:57Z-
dc.date.available2017-04-19T08:53:57Z-
dc.date.issued1994-
dc.identifier.issn1058-0468-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/3990-
dc.description.abstractPurpose: To clarify the developmental capacity of frozen two-cell blastomeres, we investigated in vivo and in vitro viabilities of blastomeres that were frozen ultrarapidly after separation from two-cell mouse embryos. Two-cell embryos obtained from superovulated F1 hybrid females were denuded by treatment with 0.5% pronase solution and then induced to separate into two single blastomeres by gentle pipetting. The blastomeres were cryopreserved by an ultrarapid freezing method. Results: The preimplantation developmental rate of two-cell embryos frozen in 3.0 M DMSO was significantly higher than the rate of those frozen in 15 and 4.5 M DMSO (at least P < 0.05). The in vitro developmental rate of the ultrarapidly frozen-thawed blastomeres separated from two-cell embryos (75.0%) was similar to that of nonfrozen blastomeres (76.0%). When eight pairs of blastocysts that developed from frozen two-cell mouse blastomeres were transferred to pregnant ICR recipients on Day 3, four live singletons were born. Conclusion: Thus, the results indicate that two-cell mouse blastomeres can be frozen by the ultrarapid freezing method.-
dc.publisherSpringer-
dc.titleCryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method-
dc.title.alternativeCryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method-
dc.typeArticle-
dc.citation.titleJournal of Assisted Reproduction and Genetics-
dc.citation.number8-
dc.citation.endPage413-
dc.citation.startPage409-
dc.citation.volume11-
dc.contributor.affiliatedAuthorMan-Jong Kang-
dc.contributor.affiliatedAuthorYong Mahn Han-
dc.contributor.affiliatedAuthorChul Sang Lee-
dc.contributor.affiliatedAuthorKyung Kwang Lee-
dc.contributor.alternativeName강만종-
dc.contributor.alternativeName한용만-
dc.contributor.alternativeName이철상-
dc.contributor.alternativeName신상태-
dc.contributor.alternativeName이경광-
dc.identifier.bibliographicCitationJournal of Assisted Reproduction and Genetics, vol. 11, no. 8, pp. 409-413-
dc.identifier.doi10.1007/bf02211728-
dc.subject.keywordfull-term development-
dc.subject.keywordtwo-cell blastomeres-
dc.subject.keywordultrarapid freezing-
dc.subject.localfull-term development-
dc.subject.localtwo-cell blastomeres-
dc.subject.localultrarapid freezing-
dc.description.journalClassY-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.