Cloning and overexpression of human immunodeficiency virus-1 gag and env derived epitopes in E. coli

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Title
Cloning and overexpression of human immunodeficiency virus-1 gag and env derived epitopes in E. coli
Author(s)
Young-Hae Chong; Mi Jin Sohn; Ji Eun Chang; Young Ik Lee
Bibliographic Citation
Molecules and Cells, vol. 4, no. 1, pp. 7-12
Publication Year
1994
Abstract
In order to develop inexpensive and sensitive serodiagnostics, parts of the gag, env genes or gag-env fusion genes of HIV-1 were cloned and expressed in E. coli, using expression vectors contaning trp or tac promoter. Immunoreacitivities of recombinant proteins were determined using HIV-1 specific antibodies in western blot analysis. Experimental results showed the feasibility of mass production of HIV-1 gag, and env derived proteins which can be used for HIV-1 serodignostics in screening a large number of blood donors to prevent HIV-1 infection.
ISSN
1016-8478
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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