Isolation of bioflocculant-producing microorganism and its culture characteristics = 응집제 생산균주의 분리 및 배양특성

Abstract

Flocculant-producing microoranisms were isolated from soil samples using kaoline as the flocculating test material. One strain that had high flocculating acitivity among them was selected and identified as arcuademdron sp. TS-49. The favorable medium for produciton of the flocculant was 3% glucose, 0.2% yeast extract, o.1% NH₄CI, o.o1% MgSO₄, and 0.05% MnSO₄ in 150ml of T.W. with intitial pH 7.0. The optimum culture temperature and pH were 30˚C and pH 7.0, respectively. The flocculant activity was observed most highly after 4 to 5 days of cultivation at the optimum condition ad decreased sigmificantly with the lapse of cultivation time. The flocculant was produced cinstituently and seemed to ve degraded for reassimilation during cultivation. The productivity achieved by this system was about tem-fold higher than that of screening medium. This bioflocculant flocculated all tested solids, including various microorganism and organic/inorganic compounds. Several qualitaive amalyses of the the bioflocculant showed that it was a kind of glycoprotein containing sugars and protein.